General description
GATOR complex protein DEPDC5 (UniProt: O75140; also known as DEP domain-containing protein 5) is encoded by the DEPDC5 (also known as KIAA0645) gene (Gene ID: 9681) in human. DEPDC5 is a component of the GATOR 1 complex that includes DEPDC5, NPRL2, and NPRL3. Its expression is observed in developing and adult brain. It acts as an inhibitor of the amino acid sensing branch of the TORC1 pathway. It acts as a negative regulator of mTOR signaling. Its DEP domain (Dsh-Egl10-pleckstrin) is localized in amino acids 1187-1262. Human cells heterozygous for DEPDC5 loss-of-function mutations are reported to be haploinsufficient for control of mTORC1 signaling. DEPDC5 +/- neurons exhibit hyperactivation of mTORC1 and enlarged somas, a phenotype that can be rescued with mTOR1 inhibition. Nonsense and missense mutation in DEPDC5 gene are known to cause familial temporal lobe epilepsy that is characterized by focal seizures arising from different cortical regions in different family members. Eight isoforms of DEPDC5 have ben described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liu, L., et al. (2020). Front. Neurosci. 14; 821; Klofas, LK., et al. (2020). Neurobiol Dis. 143; 104975).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human GATOR complex protein DEPDC5.
Application
Quality Control Testing
Evaluated by Immunocytochemistry in K562 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected DEPDC5 in K562 cells.
Tested Applications
Flow Cytometry Analysis: 0.1 µg from a representative lot detected DEPDC5 in K562 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DEPDC5 in human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound DEPDC5 peptide with a KD of 1.4 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1G17 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects GATOR complex protein DEPDC5. It targets an epitope within 19 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1G17, recombinant monoclonal |
| description | 1G17 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 181.26 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | rat, porcine, monkey, mouse, bovine, canine |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_055477 |
| UniProt accession no. | O75140 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... DEPDC5(9681) |
