General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
HLA class I histocompatibility antigen, B alpha chain (UniProt: P01889; also known as Human leukocyte antigen B, HLA-B) is encoded by the HLA-B (also known as HLAB) gene (Gene ID: 3106) in human. HLA-B is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 25-309), a transmembrane domain (aa 309-333), and a cytoplasmic domain (aa 334-363). It is an antigen-presenting major histocompatibility complex class I (MHCI) molecule that in complex with β2-microglobulin displays viral and tumor-derived peptides on antigen-presenting cells for recognition by -β T cell receptor (TCR) on HLA-B-restricted CD8+ T cells. This helps antigen-specific T cell immune response to eliminate infected or transformed cells. It typically presents intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via constitutive proteasome and interferon- -induced immunoproteasome. It contains three alpha ( 1, 2, and 3) domains. Residues 101-107 determine Bw4/Bw6 motifs, which serologically distinguish HLA-B alleles 1 domain (aa 25-114) is a structural part of the peptide-binding cleft and residues 101-107 in this region determine Bw6 motif that serologically distinguish HLA-B alleles. 2 domain (aa 115-206) is reported to mediate the interaction with Transporter Associated with Antigen Processing ( TAP1-TAP2) complex. 3 domain (aa 207-298)mediates the interaction with CD8 co-receptor. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chan, KF., et al. (2018). Nat. Commun. 9(1); 1026; Henrik N Kløverpris, HN., et al (2015). Retrovirology. 12; 20; Schmidt, J., et al. (2012). PLoS Pathog. 8(11); e1003042).
Immunogen
KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal half of human HLA-B.
Application
Quality Control Testing
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected HLA-B in Raji cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected HLA-B in lysates from Ramos cells and Human tonsil tissue and 1:1,000 dilution in A431 cells.
Affinity Binding Assay: A representative lot of this antibody bound HLA-B peptide with a KD of 7.1 x 10-8 in an affinity binding assay.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected HLA-B in Human tonsil tissue sections.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected HLA-B in one million Human peripheral blood mononuclear cells (PBMC).
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected HLA-B in Human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1L21 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects HLA-B. It targets an epitope within 25 amino acids within the extracellular domain from the C-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| description | clone 1L21 ,recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 40.46 kDa,observed mol wt ~40 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunofluorescence: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_005505 |
| UniProt accession no. | P01889 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... HLA-B(3106) |
