产品介绍
Product Description
Lambda Protein Phosphatase (Lambda PP) is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues.
One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50 mM) in 1 minute at 30°C in a total reaction volume of 50 µl.
技术参数
Specifications
| Form | Liquid |
| Storage buffer | 100 mM NaCl, 50 mM HEPES, pH 7.5, 0.1 mM MnCl2, 0.1 mM EDTA, 2 mM DTT, 0.01% Brij35, and 50% glycerol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | Remove phosphate groups from phosphorylated serine,threonine and tyrosine residues in proteins. |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Protein length | Lambda PP is cloned from Escherichia phage lambda and expressed in E.coli. |
| Nature | Recombinant |
| Predicted molecular weight | 27.32 kDa |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
| Reconstitution | |
| Alternative Names | |
| Species | Escherichia phage lambda (Bacteriophage lambda) |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note | Avoid repeated freeze/thaw cycles. For research use only. |
