产品介绍
Product Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for SARS-CoV-2 RBD has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any SARS-CoV-2 RBD present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled SARS-CoV-2 RBD antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells.Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SARS-CoV-2 RBD bound in the initial step. The color development is stopped and the intensity of the color is measured.
技术参数
Specifications
| Stability and Storage | When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃. |
| Detection method | Colorimetric |
| Sample type | Serum, Plasma and Cell culture supernatant. |
| Assay type | Quantitative |
| Sensitivity | 4.92 pg/mL |
| Range | 12.5 - 800 pg/mL |
| Recovery | 80-120% |
| Background | |
| Alternative Names | |
| Shipping | 2-8 ℃ |
| Specifications | RBD |
| Note | For Research Use Only. |
