产品介绍
Product Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative competitive enzyme immunoassay technique. Omalizumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human IgE and then pipetted into the wells. Anti-Omalizumab Neutralizing Antibody in the sample competitively binds to Omalizumab with the biotin-labeled Human IgE. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Omalizumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
技术参数
Specifications
| Stability and Storage | When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃. |
| Detection method | Colorimetric |
| Sample type | Plasma, Serum |
| Assay type | Quantitative |
| Sensitivity | 82.6 ng/mL |
| Range | 156.25 - 10,000 ng/mL |
| Recovery | 80-120% |
| Background | |
| Alternative Names | IGE25,olizumab,pSVIE26,rhuMab-E25, CAS: 242138-07-4 |
| Shipping | 2-8 ℃ |
| Specifications | Omalizumab |
| Note | For Research Use Only. |
