产品介绍
Product Description
QIAGEN PCR Cloning Kits provide ready-to-use ligation reactions, which contain linearized cloning vectors that carry U overhangs at each 3' end, allowing PCR products containing 3'-end A overhangs to be directly ligated and cloned with high efficiency and speed. The QIAGEN PCR Cloningplus Kit also provides competent E. coli cells and SOC medium for efficient transformation.
产品特点
Features
- Just 40 minutes from PCR product to plated cells
- Ready-to-use Ligation Master Mix
- High-specificity UA hybridization for efficient cloning
- Competent cells supplied with the QIAGEN PCR Cloningplus Kit
- Immediate plating of transformed competent cells
产品应用
Applications
QIAGEN PCR Cloning Kits are suitable for cloning any PCR product that has a single A overhang at each 3' end. PCR products generated using Taq and other nonproofreading DNA polymerases can be directly cloned without any preparation. Kits offering proofreading DNA polymerases, such as the HotStar HiFidelity Polymerase Kit and the QIAGEN LongRange PCR Kit, generate PCR products with A overhangs, and are also highly suited for direct use with the QIAGEN PCR Cloning Kits.
