产品介绍
Product Description
FastLane Cell RT-PCR Kits accelerate and streamline real-time RT-PCR and are ideal for experiments requiring rapid high-throughput gene expression analysis. The kits provide a set of buffers for preparing FastLane lysates from cultured cells. In addition to lysing cells, the buffers also stabilize cellular RNA and eliminate genomic DNA. FastLane lysates are used directly in real-time one-step RT-PCR and multiplex, real-time one-step RT-PCR. A variety of detection formats are available, including singleplex detection with SYBR Green or sequence-specific probes or multiplex detection with sequence-specific probes (with or without ROX passive reference dye).
产品特点
Features
- No RNA purification required, significantly saving time
- Just 3 steps from cells to real-time RT-PCR
- High-throughput analysis of 96- and 384-well plates
- Unique gDNA Wipeout Buffer enables detection of RNA only
- Immediate startup using optimized reagents and protocols
产品应用
Applications
FastLane Cell RT-PCR Kits are ideal for experiments that require rapid, high-throughput gene expression analysis, such as:
- Validation of siRNA-mediated gene knockdown
- Screening of potential drugs
- Detection of stimulated gene regulation
