产品介绍
Product Description
Purify large amounts of amplifiable DNA from hard-to-lyse formalin-fixed, paraffin-embedded (FFPE) tissue. The EZ1&2 DNA FFPE protocol uses double lysis to recover DNA effectively, while the optional uracil-N-glycosylase (UNG) step removes deaminated cytosine artifacts to limit the risk of nucleotide read errors. The EZ1&2 DNA FFPE protocols are automatable on either the EZ1 Advanced XL or the EZ2 Connect. The EZ2 Connect features cardless protocols, expanded applications and remote connectivity options. Click here to learn more about the EZ2 Connect, or contact your sales representative to discover how easily you can get an EZ2 Connect for your lab.
产品特点
Features
- High recovery of amplifiable DNA
- Paraffin removal without xylene or similar solvents
- Option to remove uracil (deaminated cytosine) artifacts
- Ready-to-use DNA for PCR, digital PCR (dPCR) and next-generation sequencing (NGS)
- Automated heating and reagent-pipetting pretreatment steps, available on the EZ2 Connect
技术参数
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, digital PCR, next-generation sequencing |
| Elution volume | 60 and 100 µl |
| Format | Magnetic bead |
| Main sample type | Formalin-fixed paraffin-embedded tissue samples |
| Processing | Automated with the EZ1 Advance XL or EZ2 Connect |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA |
| Technology | Silica technology |
| Sample amount | Tissue sections, each with a thickness of 5–10 µm, for a total volume of 4 mm3 |
产品应用
Applications
DNA isolated with the EZ1&2 DNA FFPE Kit can be used immediately for PCR, dPCR or NGS. Alternatively, it can be stored at −30 to −15°C.
