产品详情

RPMI 1640 10 X 500ML,11835055,Invitrogen

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订货号 9ZG5095
品牌型号 11835055
库存 有库存
最小订货量 1套
产品介绍 Product Description
规格
HEPES Buffer: No HEPES
Product Line: Gibco®
Serum Supplementation: Standard Serum Supplementation
Sodium Pyruvate Additive: No Sodium Pyruvate
Volume (Metric): 500 ml
Form: Liquid
Glutamine: L-Glutamine
Phenol Red Indicator: No Phenol Red
Quantity: 10 x 500 mL
储存
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
描述

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product intended use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (11835-063). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

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