规格
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Detection Method:
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Fluorescent |
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Excitation/Emission:
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553⁄570 |
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For Use With (Equipment):
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Fluorescence Microscope |
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Product Line:
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CellTracker™ |
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Shipping Condition:
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Room Temperature |
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Sub Cellular Localization:
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Cell Membranes & Lipids |
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Color:
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Yellow |
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Quantity:
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20 x 50μg |
储存
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Store in freezer (-5 to -30°C) and protect from light. |
CellTracker™ CM-DiI is a fluorescent dye well suited for monitoring cell movement or location. This dye is well retained, allowing for multigenerational tracking of cellular movements. And the red excitation/emission spectra are ideal for multiplexing with green fluorescent dyes and proteins.
Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.
• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Red excitation/emission spectra (553/570 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation
CellTracker™ CM-DiI fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into cell membrane-impermeant reaction products. CellTracker™ CM-DiI dye is retained in living cells through several generations. The dye is transferred to daughter cells, but not adjacent cells in a population. CellTracker™ CM-DiI dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ CM-DiI dye are well separated from GFP (green fluorescent protein) spectra allowing for multiplexing.
