规格
|
|
Kinase Group:
|
Serine/Threonine Kinase |
|
Species:
|
Human |
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Validated Application:
|
Kinase Assay |
|
Molecular Weight:
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63.6/38/65 kDa |
|
Quantity:
|
100μg |
|
Protein Length:
|
aa 10-end |
|
Protein Tag:
|
His Tag |
储存
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Storage Buffer: 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol.
Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws. |
AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the gamma subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKβ. Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags.
• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen® Eu Kinase Binding Assay
