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规格 |
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| Bacterial or Yeast Strain: | TOP10 |
| Cloning Method: | TOPO®-TA |
| Product Line: | One Shot™ |
| Shipping Condition: | Dry Ice |
| Vector: | TOPO-TA Cloning Vectors |
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储存 |
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Box 1: • Topoisomerase I-activated pCR™II-TOPO® vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot® Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C. |
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描述 |
The TOPO® TA Cloning® Dual Promoter Kit is for fast, efficient cloning and subsequent in vitro transcription/translation. This kit includes the pCR™II-TOPO® TA vector (see figure). By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable method, taking only 5 minutes using a 3-step protocol, and yielding up to 95% recombinants. Convenient features of the TOPO® TA Cloning® Dual Promoter Kit include:
• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• T7 and SP6 promoters for efficient in vitro transcription
• M13 forward and reverse primer sites for sequencing
• 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
• Kanamycin and ampicillin resistance for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
• Includes OneShot® TOP10 Competent cells for added convenience and highest cloning efficiencies
This product comes in Supply Center packaging, which features an additional outer box that holds both the TOPO® vector box and the competent cells box. This special packaging helps ensure that visitors to a Supply Center obtain the complete kit.
