规格
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Cloning Method:
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Restriction Enzyme ⁄ MCS |
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Delivery Type:
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Transfection |
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Key Functions:
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Reporter Assays |
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Product Line:
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Pierce™ |
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Promoter:
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TK |
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Reporter Gene:
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Green Renilla Luciferase |
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Selection Agent (Eukaryotic):
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Puromycin |
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Description:
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pTK-Green Renilla Luc Vector |
储存
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Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C. |
The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.
Features of the pTK-Green Renilla Luc vector:
• Weak (TK) constitutive promoter for co-transfection and normalization
• Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin
More Product Data
• Luciferase assays in hard-to-transfect Jurkat cells
Related Products
pMCS-Green Renilla Luc Vector for Luciferase Assays
pCMV-Green Renilla Luc Vector for Luciferase Assays
