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规格 |
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| Cloning Method: | Gateway® |
| Constitutive or Inducible System: | Constitutive |
| Delivery Type: | Lentiviral |
| Key Functions: | Viral Expression |
| Product Line: | Gateway®, HiPerform™, ViraPower™ |
| Promoter: | CMV |
| Protein Tag: | V5 Epitope Tag |
| Selection Agent (Eukaryotic): | Blasticidin |
| Selection Marker Promoter: | SV40 Promoter |
| Vector: | pDEST, pLenti |
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储存 |
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Vector Kit (Box 1) pLenti6.3⁄V5-DEST™ (150 ng⁄µL): 6 µg, store at -20°C pLenti6.3⁄V5-GW⁄lacZ Control vector (500 ng⁄µL): 10 µg, store at -20°C One Shot® Stbl3™ Chemically Competent E. coli (Box 2) Stbl3™ Cells: 21 x 50 µL, store at -80°C pUC19 control DNA (10 pg⁄µL): 50 µL, store at -80°C S.O.C. Medium: 6 mL, store at -80°C |
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描述 |
Invitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway® Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit (catalog K5330-00).
The pLenti6.3⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway® recombination cloning technology
Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
• Blasticidin selection
Kit includes
• A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ
• Stbl3™ competent cells
For research use only. Not intended for any therapeutic or diagnostic use.
