规格
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Accession Number:
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NP_005219.2 |
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Protein Form:
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Recombinant, Active, Cytoplasmic, Mutant |
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Shipping Condition:
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Dry Ice |
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Molecular Weight:
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84.2 kDa |
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Validated Application:
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Kinase Assay |
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Expression System:
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Insect |
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KinaseFamily:
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EGF Receptors (ErbBs) |
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Protein Length:
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aa 695 - end |
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Protein Tag:
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GST Tag |
储存
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Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol |
This EGFR (ErbB1) T790M C797S L858R recombinant human protein (695–end) was expressed in insect cells. EGFR receptor tyrosine kinase is a transmembrane glycoprotein. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival. Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms. Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation. EGFR (ErbB1) T790M C797S L858R recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.
• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service
