规格
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Chemical Reactivity:
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Thiol |
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Description:
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DyLight 633 Maleimide |
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Excitation/Emission:
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638⁄658 |
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Product Line:
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DyLight™ |
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Label Type:
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DyLight Fluors |
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Label or Dye:
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DyLight™ 633 |
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Reactive Moiety:
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Maleimide |
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Solubility:
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DMF (Dimethylformamide) |
储存
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Store desiccated at -20°C. Shipped at ambient temperature. |
Thermo Scientific DyLight 633 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 633 used to fluorescently label sulfhydryl-containing peptides, proteins, and other biomolecular probes.
DyLight 633 fluoresces red and has physical properties comparable to other 633 dyes, including Alexa Fluor™ 633, over a broad pH range (pH 4–9). The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.
Features of DyLight 633 Maleimide:
• High performance—DyLight 633 shows brighter fluorescence than Alexa Fluor 633
• Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
• Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
• Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency
Applications:
• Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies
DyLight 633 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5–7.5. Learn more about maleimide chemistry.
