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规格 |
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| Accession Number: | NM_030760.3 |
| Assay Entry: | Cell-based beta-arrestin recruitment |
| Cell Line: | U2OS |
| Cell State: | Dividing Cells |
| Cytoplasmic Tail: | Tail from AVPR2 GPCR |
| Druggable Target: | GPCRs |
| Gene Symbol: | EDG8 |
| Population Status: | Pool of Stable Cells |
| Product Line: | GeneBLAzer®, Tango™ |
| Readout: | End Point |
| Reporter Gene: | BLA (Beta-Lactamase) |
| System Type: | Tango™ |
| Target Entry: | EDG8 |
| Technique: | FRET |
| Detection Method: | Fluorescent |
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储存 |
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Contents: K1518 Tango™ EDG8-bla U2OS cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • Tango™ EDG8-bla U2OS cells • Tango™ EDG8-bla U2OS Assay Protocol • Certificate of Analysis Storage: The Tango™ EDG8-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
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描述 |
The Tango™ EDG8-bla U2OS cells contain the human Endothelial Differentiation Gene 8 (EDG8) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ EDG8-bla U2OS cells are functionally validated for Z' and EC50 concentrations of S1P. In addition, Tango™ EDG8-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
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