规格
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Form:
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Powder |
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Quantity:
|
100mg |
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Shipping Condition:
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Approved for shipment at Room Temperature or on Wet Ice |
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Concentration:
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>40 U/mg |
储存
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One vial
Store in refrigerator (2–8°C). |
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:
• Over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At comparatively high temperatures—optimum digestion temperature is 65°C
Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.
