Clone REAL414 is an antibody fragment derived from the full Gr-1 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL414 recognizes the mouse Gr-1 antigen (granulocyte-differentiation antigen-1), also known as Ly-6G, a 21-25 kDa, GPI-anchored cell surface protein. Cross-reactivity of Anti-Gr-1 with Ly-6C was not detected on hematopoietic cells that express Ly-6C and are negative for Ly-6G.
Gr-1 is expressed on mature granulocytes in bone marrow and peripheral tissues. The Anti-Gr-1 antibody also stains monocytes transiently during their differentiation in bone marrow and at low levels plasmacytoid dendritic cells in lymphoid tissues.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).
Ly-6G, Ly-6G.1
Technical specifications
