产品介绍
Product Description
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规格 |
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| Excitation/Emission: | FITC: 494⁄518, PI: 535⁄617 |
| Flow Cytometer Laser Lines: | 488 |
| Label Type: | Classic Dyes |
| Label or Dye: | propidium iodide, FITC (Fluorescein) |
| No. of Reactions: | 50 |
| Product Line: | Vybrant® |
| Shipping Condition: | Wet Ice |
| Detection Method: | Fluorescent |
| For Use With (Equipment): | Flow Cytometer |
| Format: | Tube(s) |
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储存 |
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Contains 1 vial of annexin V, FITC conjugate (250 µL ), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL). Store in refrigerator (2–8°C) and protect from light.Store in refrigerator (2–8°C) and protect from light. |
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描述 |
This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent FITC dye and dead cells using propidium iodide (PI). Propidium iodide stains necrotic cells with red fluorescence. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence.
View a selection guide for all apoptosis assays for flow cytometry.
