The Adult Neuron Isolation Kit, mouse depletes all non-neuronal cells to acquire highly purified neurons from mouse brain tissue older than postnatal day 7, resulting in superior performance in single cell sequencing and cell culture.
The Adult Neuron Isolation Kit, mouse is an indirect magnetic labeling system for the isolation of untouched neurons from cell suspensions of mouse neural tissue older than postnatal day 7 (P7). Non-neuronal cells like astrocytes, oligodendrocytes, microglia, endothelial cells, fibroblasts, except erythrocytes, are indirectly magnetically labeled by using biotin-conjugated antibodies specific for non-neuronal cells in combination with Anti-Biotin MicroBeads. Isolation of highly pure unlabeled neuronal cells is achieved by depletion of the magnetically labeled cells.
The cell number and composition of the neuronal cell fraction varies with the mouse age and the brain region used for cell isolation.
For optimal results, it is recommended to use the Adult Brain Dissociation Kit, mouse and rat (# 130-107-677) prior to this kit.
