规格
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Exonuclease Activity:
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3' - 5' |
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Fidelity (vs. Taq):
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26 X |
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GC-Rich PCR Performance:
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Low |
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Hot Start:
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Built-In Hot Start |
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Overhang:
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Blunt |
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Polymerase:
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AccuPrime™ Pfx DNA Polymerase |
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Product Line:
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AccuPrime™ |
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Reaction Format:
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SuperMix or Master Mix |
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Reaction Speed:
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Standard |
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Shipping Condition:
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Approved for shipment on Wet or Dry Ice |
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Size (Final Product):
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15 kb or less |
储存
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• 4 × 1.125 mL AccuPrime Pfx SuperMix
Store at -20°C in a non-frost-free freezer. |
AccuPrime™ Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accesory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half. With AccuPrime™ Pfx SuperMix you will:
• Achieve >26-times higher fidelity than with Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimization
• Produce products with blunt ends
Using AccuPrime™ Pfx SuperMix
AccuPrime™ Pfx SuperMix is ideal for high-fidelity, high-specificity amplification of DNA fragments for applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. Thermostable AccuPrime™ proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.
