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规格 |
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| Product Line: | Pierce™ |
| Substrate Properties: | Chemical Substrate |
| Substrate Type: | AP (Alkaline Phosphatase) Substrate |
| Description: | Pierce PNPP Substrate Kit |
| Detection Method: | Colorimetric |
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储存 |
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Sufficient For: 5mL of typical 1X substrate solution per tablet (525 mL total; approx. 5250 wells at 100 µL per well) • Diethanolamine Buffer, 225 mL • PNPP Tablets, 105 tablets (5 mg/tablet) Store at 4°C. |
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描述 |
Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. Our Phosphatase Substrate Kit contains PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Part No. 34064) is also provided individually as a 5X concentrate. The Pierce formulation has an optimal and consistent pH and it is stable even at a 1X concentration.
Features of PNPP (p-nitrophenyl phosphate):
• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative
PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. The substrate is offered in several formats.
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