规格
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Cloning Method:
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Restriction Enzyme ⁄ MCS |
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Delivery Type:
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Transfection |
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Key Functions:
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Reporter Assays |
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Product Line:
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Pierce™ |
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Promoter:
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minP |
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Reporter Gene:
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TurboLuc™16 Luciferase |
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Selection Agent (Eukaryotic):
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Hygromycin |
储存
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Store in freezer (-5 to -30°C). |
The pMCS minP-Tluc16 Hygro Vector is a transcriptional reporter vector that encodes the smallest known luciferase, TurboLuc™16 (Tluc16) luciferase, as the reporter. The vector contains a multiple cloning site (MCS) and a core minimal promoter (minP) upstream of the reporter gene for the insertion of regulatory elements lacking a promoter activity.
• Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systems
• Optimized minimal core promoter (minP) and 5′ UTR region for efficient expression of Tluc16 luciferase
• Multiple cloning sites provide versatility for transfer of regulatory elements from one vector to another
• Synthetic polyA terminator and Transcriptional Pause Site (TPS) included upstream of MCS to minimize non-specific transcriptional read-through
• Hygromycin resistance gene for use as a selection marker to generate mammalian stable cell lines
Tluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family. The wild-type luciferase has been modified to reduce its size, increase its brightness, and enable its intracellular expression.
The pMCS minP-Tluc16 Hygro luciferase expression vector contains a synthetic polyA terminator and a Transcriptional Pause Site (TPS) upstream of the MCS to minimize non-specific transcriptional read-through. This vector carries the hygromycin resistance gene which can be used as a mammalian selection marker to generate stable cell lines. The Tluc16 luciferase activity can be measured using the Thermo Scientific TurboLuc™ One-Step Glow Assay Kit.
