规格
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Assay:
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Serine⁄Threonine Kinase Assay |
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Assay Entry:
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Biochemical phospho-specific activity (no antibody) |
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Excitation/Emission:
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405⁄465, 535 |
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No. of Reactions:
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800 Reactions |
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Product Line:
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Z´-LYTE® |
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Readout:
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End Point |
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References:
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JBC(2001) 276, p38956 |
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Shipping Condition:
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Dry Ice |
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Source:
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myosin light chain 2 (KKRPQRRYSNVF) |
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Substrate Properties:
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Peptide-Based Substrate |
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Target Entry:
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ROCK2, MYLK2, DAPK3, CDC42BPA, CDC42BPB, CAMK4 |
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Target Enzyme:
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Serine⁄Threonine Kinases |
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Technique:
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FRET |
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Validated Kinases:
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CAMK4, CDC42BPA, CDC42BPB, DAPK3, MYLK2, ROCK2 |
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Label or Dye:
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Methylcoumarin |
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Format:
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384-well plate |
储存
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Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. |
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon myosin light chain 2, containing Ser-20 and a Hyd-X-R-X-X-(S⁄T) consensus sequence, where Hyd is a hydrophobic residue and X is any residue.
