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规格 |
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| Accession Number: | NM_000621.2 |
| Assay Entry: | Cell-based beta-arrestin recruitment |
| Cell Line: | U2OS |
| Cell State: | Division-Arrested Cells |
| Cytoplasmic Tail: | Tail from AVPR2 GPCR |
| Druggable Target: | GPCRs |
| Gene Symbol: | HTR2A |
| Population Status: | Single Cell Clone Origin |
| Product Line: | GeneBLAzer® |
| Protein Family: | GPCR (G-Protein Coupled Receptors) |
| Readout: | End Point |
| Reporter Gene: | BLA (Beta-Lactamase) |
| System Type: | Tango™ |
| Target Entry: | HTR2A |
| Detection Method: | Fluorescent |
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储存 |
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K1613 Tango™ HTR2A-bla U2OS DA Assay Kit Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • Tango™ HTR2A-bla U2OS DA cells • LiveBLAzer™-FRET B⁄G Loading Kit, 70µg • Solution D • Tango™ HTR2A-bla U2OS DA Assay Protocol • LiveBLAzer™-FRET B⁄G Loading Protocol • Certificate of Analysis The Tango™ HTR2A-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
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描述 |
The Tango™ HTR2A-bla U2OS DA cells contain the human Serotonin Type 2A receptor (HTR2A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ HTR2A-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ HTR2A-bla U2OS DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
