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规格 |
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| Final Product Type: | Transcriptome RNA |
| For Use With (Application): | Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction |
| Isolation Technology: | Magnetic Bead |
| Quantity: | 1 each |
| Sample Type: | Total RNA |
| Yield: | 1 µg |
| High-throughput Compatibility: | High-throughput Compatible |
| Purification Target: | Transcriptome RNA |
| Sequencing Type: | Transcriptome Sequencing |
| Shipping Condition: | Wet Ice |
| Starting Material Cell No.: | 2-10 µg total RNA |
| Starting Material Amount: | 2-10 µg Total RNA |
| Starting Material Volume: | 20 µl |
| System Type: | RiboMinus |
| Product Line: | RiboMinus™ |
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储存 |
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Each kit contains: • 6 ml RiboMinus™ Magnetic Beads • 80 µl RiboMinus™ Eukaryote Probe (15 pmol/µl) • 10 ml Hybridization Buffer • 12 ml DEPC-treated (RNase-free) water Store at 4°C. |
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描述 |
The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.
The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:
• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology
Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module.
