规格
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Label Type:
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Other Label(s) or Dye(s) |
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Label or Dye:
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AMC (7-amino-4-methylcoumarin) |
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Product Line:
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EnzChek® |
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Shipping Condition:
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Room Temperature |
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Detection Method:
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Fluorescent |
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For Use With (Equipment):
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Fluorometer, Microplate Reader |
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Format:
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Cuvettes, 96-well plate |
储存
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Store in freezer (-5 to -30°C) and protect from light. |
The EnzChek Caspase-3 Assay Kit #1 allows detection of apoptosis by providing a simple and reliable method for assaying caspase-3 activity. The basis for the assay is the aminomethylcoumarin (AMC)-derived substrate Z-DEVD-AMC. This substrate, which is weakly fluorescent in the UV range (excitation/emission maxima ~330/390 nm), yields a bright, blue-fluorescent product (excitation/emission maxima ~342/441 nm) upon proteolytic cleavage. The kit can be used to continuously measure the activity of caspase-3 and closely related proteases in cell extracts and purified enzyme preparations, using a fluorometer or fluorescence microplate reader.
