产品介绍
Product Description
QIAsymphony Bisulfite Kits provide a fast, streamlined procedure for efficient bisulfite conversion and purification of DNA prepared from formalin-fixed, paraffin-embedded (FFPE), blood, cell, or tissue samples. The kits enable highly efficient conversion of unmethylated cytosines to uracils in as little as 30 minutes while offering outstanding flexibility in choice of starting material. Using the QIAsymphony SP instrument, isolate high-quality genomic DNA that is suitable for direct use in downstream applications, such as Pyrosequencing, PCR or NGS applications, or for storage for later use.
产品特点
Features
- Fast and streamlined combined bisulfite conversion and DNA purification
- Directly isolate purified, bisulfite-converted DNA without intermediary steps
- Produces high-quality, bisulfite-converted DNA suitable for pyrosequencing, PCR or NGS applications
- High cytosine conversion rates of over 99%
产品应用
Applications
The QIAsymphony Bisulfite Kits deliver fast and efficient bisulfite conversion of DNA from various starting material that is highly suited for all techniques currently used for the analysis of DNA methylation, including:
- PCR: end-point, real-time and methylation-specific
- Bisulfite sequencing (direct and cloning)
- NGS
- Pyrosequencing
- COBRA (Combined Bisulfite Restriction Analysis)
