规格
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Chemical Reactivity:
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Carboxylic Acid, Ketone, Aldehyde |
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Label Type:
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Classic Dyes |
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Label or Dye:
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Cascade Blue® |
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Product Line:
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CASCADE BLUE® |
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Reactive Moiety:
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Amine, Hydrazide |
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Shipping Condition:
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Room Temperature |
储存
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Store at room temperature and protect from light. |
The cell-impermeant, fixable, polar tracer Cascade Blue® hydrazide can be used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. This water-soluble tracer can be introduced into cells by whole-cell patch clamping, iontophoresis, osmotic lysis of pinocytic vesicles or comparable methods. The primary aliphatic amine of Cascade Blue® hydrazide can also be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3). Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC (E2247).
