规格
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Label Type:
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Conjugating Groups |
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Label or Dye:
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Carboxyl Group, Amine |
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Product Line:
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Pierce™ |
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Reactive Moiety:
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Amine, Primary Amine |
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Spacer:
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Cleavable, Mid-Length |
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Chemical Reactivity:
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Amine, Carboxylic Acid |
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Solubility:
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Water |
储存
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Store at 4°C protected from moisture. Shipped at ambient temperature. |
Thermo Scientific Pierce AEDP is an amine-and-carboxyl compound with an internal disulfide bond. AEDP enables construction, extension and modification of several functional groups on proteins and surfaces.
Features of AEDP:
• Use as a spacer arm in conjugation schemes
• Use for reversible immobilization applications
• Cleave the internal disulfide bond with reducing agents
• Incorporate amine or carboxyl groups into proteins/peptides using crosslinking agents
• React the amine end with amine-reactive acylation agents
• Use with water-soluble carbodiimide (EDC) to conjugate to amines or carboxylates
AEDP (3-[(2-aminoethyl)dithio]propionic acid·HCl) is a cleavable amino acid that can be reacted with amine-reactive acylation agents to create amide bonds. Alternatively, the carbodiimide EDC can be used to conjugate AEDP to molecules containing amines or carboxylates, which results in some degree of polymerization. Reducing agents, such as DTT and TCEP, are effective for cleaving the disulfide bond.
