规格
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Assay Entry:
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Cell-based beta lactamase reporter gene |
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Cell State:
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Dividing Cells |
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Druggable Target:
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Cytokines, Signaling Pathway |
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Product Line:
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CellSensor™ |
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System Type:
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CellSensor™ |
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Target Entry:
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JAK/STAT (Type I IFN) pathway, TYK2, JAK1 |
储存
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CellSensor® Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use. |
Interferon cell signaling is a critical pathway involved in viral defense and autoimmune disease. The CellSensor® ISRE-bla HEK 293T cell line is responsive to interferon alpha and can be used to probe the JAK/STAT signaling pathway.The CellSensor™ ISRE-bla HEK 293T cell line contains the β-lactamase reporter gene under the control of the interferon stimulated response element (ISRE). This cell line was engineered by transduction of the ISRE-bla construct into HEK 293T cells by lentivirus. Flow cytometry was then used to isolate a pool of clones responsive to interferon alpha a stimulation. A dose response curve for this assay against interferon alpha is available in the validation packet. Academic and non-profit customers, please inquire for special pricing.
