规格
|
|
Detection Method:
|
Biotin-Based |
|
Final Product Type:
|
DNA |
|
No. of Reactions:
|
10 |
|
Includes Label or Dye:
|
Yes |
|
Labeling Method:
|
Direct Labeling |
|
Labeling Target:
|
Genomic DNA, PCR Products, DNA (General) |
|
Product Line:
|
DecaLabel |
Thermo Scientific Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.
Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin detection systems.
Highlights
• Non-radioactive labeling of DNA
• Efficient priming of labeling reactions with random decamers
• High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction
Applications
• Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots, and in situ hybridizations.
Principle
Random decamers are annealed to a denatured template DNA molecule, and new strands are synthesized by the Klenow Fragment, exo- in the presence of biotin-dUTP. During this reaction, the biotinylated nucleotides are incorporated into the newly synthesized complementary DNA strand.
Related Products
Biotin DecaLabel DNA Labeling Kit
