规格
|
|
Concentration:
|
200 U⁄µl |
|
Format:
|
Tube(s) |
|
Includes:
|
SuperScript II Reverse Transcriptase (2,000 units total, at 200U/μL), 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and 100mM DTT |
|
No. of Reactions:
|
10 Reactions |
|
Quantity:
|
2000 units |
|
Enzyme Function:
|
RNA dependent DNA Polymerase |
|
Size (Final Product):
|
12.3 kb or less |
|
Form:
|
Frozen |
|
Optimal Reaction Temperature:
|
42° C |
|
Reverse Transcriptase:
|
SuperScript™ II |
|
Ribonuclease H Activity:
|
Reduced |
|
Shipping Condition:
|
Wet Ice |
|
Product Line:
|
SuperScript™ |
储存
|
|
SuperScript™ II Reverse Transcriptase (2,000 units total, at 200U/µL) is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT. |
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA.
SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.
Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.
