规格
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Cloning Method:
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Restriction Enzyme ⁄ MCS |
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Key Functions:
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Subcellular Localization |
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Product Line:
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pShooter™ |
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Constitutive or Inducible System:
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Constitutive |
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Delivery Type:
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Transfection |
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Promoter:
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CMV |
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Selection Agent (Eukaryotic):
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Geneticin® (G-418) |
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Vector:
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pCMV |
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Protein Tag:
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c-Myc Epitope Tag |
储存
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20 µg of the pShooter™ vector and GFP control are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored. |
The pShooter™ vectors are designed to localize recombinant proteins to the nucleus, mitochondria, endoplasmic reticulum, or cytoplasm. In addition to the localization signal, each pShooter™ vector contains:
• Strong mammalian promoter (CMV or EF-1É) for constitutive expression of your protein of interest
• C-terminal c-myc epitope for detection with an Anti-myc Antibody
• Bovine Growth Hormone (BGH) polyadenylation site for mRNA stability
• f1 origin for single-stranded rescue of sense DNA
• Neomycin resistance gene for selection of mammalian cells with Geneticin¤ selection agent
• Ampicillin resistance and pUC origin for selection and maintenance in E. coli
Each vector is provided with a positive control plasmid. The positive control expresses GFP and targets it to the same subcellular location as the pShooter™ vector.
