规格
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Fidelity (vs. Taq):
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1 X |
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GC-Rich PCR Performance:
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Low |
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Hot Start:
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Built-In Hot Start |
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Overhang:
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3'-A |
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Polymerase:
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Tfi DNA Polymerase |
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Product Line:
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Platinum® |
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Reaction Format:
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Separate Components |
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Reaction Speed:
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Standard |
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Shipping Condition:
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Dry Ice |
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Size (Final Product):
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1 kb or less |
储存
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Platinum® Tfi DNA Polymerase (500 units total, at 5U/µL) is supplied with 5X Platinum® Tfi Reaction Buffer (4 × 1.3 mL) and 50 mM Magnesium Chloride (1 mL). Store at -20°C (non-frost-free). |
Platinum® Tfi Exo(-) DNA Polymerase is recombinant Tfi Exo(-) DNA Polymerase complexed with a proprietary antibody mix that inhibits polymerase activity at ambient temperature, allowing room-temperature reaction setup. Activity is restored after the initial denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' for increased specificity, sensitivity, and yield. Tfi Exo(-) DNA Polymerase is purified from E. coli. expressing cloned mutants of the Thermus filiformis DNA polymerase gene. This enzyme has 5'→3' polymerase activity, but lacks both 5'→3' and 3'→5' exonuclease activity for improved yield and robustness. Tfi Exo(-) DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer.
Applications
Platinum® Tfi Exo(-) DNA Polymerase can be used in protocols that currently use Platinum® Taq DNA Polymerase without modification. PCR performance is comparable to that of Platinum® Taq in yield, specificity, fidelity, and robustness. Like Platinum® Taq, Platinum® Tfi Exo(-) DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3' ends of PCR products.
