规格
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Activity:
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0.5 ng/ml |
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Bioassay:
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Proliferation of OCI-AML5 cells |
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Endotoxin Level:
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< 0.1 ng/µg |
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Product Line:
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CTS™ |
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Purity or Quality Grade:
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Carrier-Free |
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Shipping Condition:
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Wet Ice |
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Expression System:
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Mammalian |
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Protein Family:
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Growth Factors & Receptors |
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Protein Form:
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Full Length, Recombinant, Ligand |
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Protein Subtype:
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FLT-3 Ligand |
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Research Category:
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Clinical Research, Stem Cell Research, Signal Transduction, Oncology |
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Species:
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Human |
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Validated Application:
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Cell Culture |
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Molecular Weight:
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30 kDa |
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Purification Method:
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Sequential Chromatography |
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Purity:
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95 % |
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Gene Alias:
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FLT-3 Ligand |
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Protein Length:
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158 aa |
Cell Therapy Systems CTS™ Recombinant Human FLT3 ligand is a lyophilized bioactive protein intended for use in cell culture applications, including pre-clinical and clinical cell therapy studies. FLT3L (fms-like tyrosine kinase-3 ligand) is a cytokine involved in hematopoeisis. The FLT3 Ligand CTS™ Recombinant Human is expressed in HEK-293 cells and verified for activity through dose-dependent proliferation of human OCI-AML5 cells.
• Highly Pure—minimizes interference from other proteins or contaminants
• Active—get more results with less protein
High Purity
To help verify that GIBCO® CTS™ Recombinant growth factors are of the highest quality, each protein is analyzed for purity, along with structural homogeneity, to help ensure a biologically active protein. The purity of each GIBCO® CTS™ recombinant growth factor is verified by SDS-PAGE to be over 95% pure and to have endotoxin levels below 0.1 ng/μg.
High Biological Activity
All GIBCO® CTS™ Recombinant growth factors are verified for activity. Each lot has its own ED50 determined using an activity assay based on a specific function of the growth factor. The activity of our FLT3 Ligand CTS™ Recombinant Human growth factor is determined by the dose-dependent proliferation of human OCI-AML5 cells to have an ED50 range from 0.5–1.0 ng/mL (specific activity: 2.0 × 106–1.0 × 106 units/mg). Optimal concentration for individual applications should be determined by a dose response assay.
Related Links
• Learn More About GIBCO® Cell Therapy Systems (CTS™) Products
• Cell Therapy Systems Technical Support Web Page
• Our Full Collection of GIBCO® CTS™ Growth Factors
• Our Full Collection of GIBCO® Growth Factors
