规格
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Binding Property:
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Standard Capacity, Affinity |
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Plate Blocking:
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SuperBlock™ Buffer |
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Plate Type:
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Detection Plate, Immunoassay, ELISA |
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Product Line:
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NeutrAvidin™, Pierce™ |
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Color:
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Black |
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Detection Method:
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Fluorescent |
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Format:
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384-well plate |
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Surface Coating:
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NeutrAvidin™ Protein |
储存
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Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C. Plates are shipped at ambient temperature. |
Thermo Scientific NeutrAvidin Protein Coated Black 384-Well Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.
Features of NeutrAvidin Protein Coated Black 384-Well Plates:
• Black plates for fluorescent detection methods
• Pre-blocked with SuperBlock Blocking Buffer
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins
Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.
Related Products
Pierce™ NeutrAvidin™ Coated Plates, White, 384-Well
