规格
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Accession Number:
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NP_173354 |
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Kinase Group:
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Serine/Threonine Kinase |
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Protein Form:
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Recombinant, Active, Catalytic |
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Research Category:
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Drug Development, Signal Transduction |
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Species:
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Human |
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Gene ID (Entrez):
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150094 |
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Molecular Weight:
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61 kDa |
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Gene Alias:
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SIK1, SNF1LK, MSK |
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Validated Application:
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Kinase Assay |
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Expression System:
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Baculovirus |
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Protein Length:
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aa 1-303 |
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Protein Tag:
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GST Tag |
储存
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Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol
Store at -68 to -85°C. After first use, aliquot and store to avoid multiple freeze-thaws. |
SIK1 is a protein kinase that is involved in regulating AMPK-related kinases. This recombinant human SIK1 (1-303) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
SIK1 may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK1 is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4, and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane. This results in an increase in intracellular sodium in intact mammalian cells.
Learn more about our kinase proteins >
Related Links:
LanthaScreen® Kinase Activity Assay
LanthaScreen® Eu Kinase Binding Assay
