规格
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Final Product Type:
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Genomic DNA |
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For Use With (Application):
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PCR, Southern Blotting, Sequencing, Cloning |
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Purification Target:
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Genomic DNA |
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Sample Type:
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Blood |
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Isolation Technology:
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Organic Extraction |
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High-throughput Compatibility:
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Not High-throughput Compatible (Manual) |
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Shipping Condition:
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Room Temperature |
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Product Line:
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DNAzol® |
储存
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Store at room temperature. |
DNAzol® BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes.
• Specifically formulated for extraction of genomic DNA from whole blood
• Optimized for the rapid Isolation of genomic DNA
Efficient Isolation of Genomic DNA from Whole Blood
DNAzol® BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol® BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. 1 ml of DNAzol® Reagent can be used to isolate genomic DNA from 0.5 ml of whole blood to yield 10-20 µg DNA. In addition to the isolation of genomic DNA, DNAzol® BD Reagent can also be used for the isolation of apoptotic fragments from whole blood and viral DNA from serum.
Rapid Isolation of Genomic DNA
The DNAzol® Reagent combines reliability, efficiency and simplicity in its isolation protocol. In the procedure, blood samples are mixed with DNAzol® Reagent and DNA is precipitated from the resulting lysate with isopropanol. The DNA pellet is then washed successively with DNAzol® Reagent followed by ethanol, and then solubilized. The entire procedure can be completed in about 30 min.
Isolated DNA can be Used for a Number of Downstream Applications:
Genomic DNA extracted using the DNAzol® procedure is suitable for a variety of applications, including Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
