产品介绍
Product Description
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规格 |
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| Bacterial or Yeast Strain: | DH5α |
| Blue/White Screening: | Yes |
| Cloning Methylated DNA: | Yes |
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
| Competent Cell Type: | Electrocompetent |
| F'episome (to make ssDNA): | Lacks F' Episome |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Plasmid: | May be used for plasmids > 20 kb |
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| Product Line: | DH5a, ElectroMAX™ |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| Reduces Recombination: | Yes |
| Shipping Condition: | Dry Ice |
| T1 Phage - Resistant (tonA): | No |
| Transformation Efficiency: | > 1x10^10 |
| Transformation Efficiency Level: | High Efficiency (> 10^9 cfu⁄µg) |
| Format: | Tube(s) |
| Improves Plasmid Quality: | Yes |
| Species: | E. coli |
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储存 |
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Contains: • ElectroMAX DH5α-E Competent Cells: 5 vials, 100 µl each • pUC19 DNA (10 pg/µl): 1 vial, 50 µl • S.O.C. medium: 2 bottles, 6 ml each Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |
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描述 |
ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation. They may be used in procedures requiring high transformation efficiencies, such as generation of cDNA libraries or in transformations with limited input DNA. ElectroMAX DH5α-E cells offer:
• >1 x 1010 transformants/µg efficiency with electroporation
• Greatly increased plasmid yield and quality due to endA1 mutation
• High-efficiency transformation with plasmids 30 kb in size
• Blue/white screening of recombinant clones due to lacZΔM15
• Ensured insert stability due to recA1 mutation
