QIAamp DNA Blood Kits
For purification of genomic, mitochondrial or viral DNA from blood and other body fluids
Features
Rapid purification of high-quality, ready-to-use DNA
No organic extraction or alcohol precipitation
Consistent, high yields
Complete removal of contaminants and inhibitors for reliable results
Kit formats for low- to high-throughput – options for automation of all kits
QIAamp DNA Blood Kits provide silica-membrane-based DNA purification from whole blood, plasma, serum and other body fluids. The kits are designed for a range of sample sizes from 200 μl up to 10 ml fresh or frozen human whole blood. QIAamp spin columns can be easily processed in a centrifuge or on vacuum manifolds. A convenient 96-well format using centrifugation enables purification of DNA for labs that need high-throughput DNA purification from blood, buffy coat, plasma, serum, bone marrow, lymphocytes and body fluids. A dedicated kit is also available for automated purification of 1–12 samples on the QIAcube Connect.
QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure "Apoptotic banding in stored blood"). The purified DNA is suitable for long-range PCR amplification (see figure " Long-range PCR") and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure " Paternity testing by RFLP analysis").
The QIAamp DNA Blood Midi Kit yields up to 94.5% recovery of DNA; the QIAamp DNA Blood Maxi Kit yields up to 95.8% recovery of DNA, depending on the starting cell densities (see table “DNA yields from human whole blood with different cell densities”).
DNA yields from human whole blood with different cell densities
| Leukocytes per ml | DNA yield (µg) | DNA recovery (%) | ||
|---|---|---|---|---|
| QIAamp Midi | QIAamp Maxi | QIAamp Midi | QIAamp Maxi | |
| 2.5 x 105 | 3.0 | 15.8 | 92.3 | 95.8 |
| 1.0 x 106 | 11.0 | 60.4 | 91.7 | 91.5 |
| 5.0 x 106 | 62.4 | 312.0 | 94.5 | 94.5 |
| 1.0 x 107 | 116.3 | 624.6 | 88.1 | 94.6 |
Comparison of QIAamp DNA Blood Kits
| Features | QIAamp DNA Blood Mini Kit | QIAamp DNA Blood Midi Kit | QIAamp DNA Blood Maxi Kit | QIAamp DNA Blood Maxi Kit |
|---|---|---|---|---|
| Applications | PCR, long-range PCR, Southern blotting | PCR, Southern blotting | PCR, blotting | Southern blotting, genome mapping |
| Elution volume | 50–200 µl | 100–400 µl | 500–2000 µl | 50–200 µl |
| Format | Spin column | Spin column | Spin column | 96-well plate |
| Main sample type | Whole blood, body fluids | Whole blood, body fluids | Whole blood, body fluids | Whole blood, body fluids |
| Processing | Manual (centrifugation or vacuum) | Manual (centrifugation or vacuum) | Manual (centrifugation or vacuum) | Manual (centrifugation or vacuum) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA, mitochondrial DNA, viral DNA | Genomic DNA, mitochondrial DNA, viral DNA | Genomic DNA, mitochondrial DNA, viral DNA | Genomic DNA, mitochondrial DNA, viral DNA |
| Sample amount | 1–200 µl | 0.3–2 ml | 3–10 ml | <200 µl |
| Technology | Silica technology | Silica technology | Silica technology | Silica technology |
| Time per run or per prep | 20–40 minutes | 55 minutes | 55 minutes | 2–3 hours (192 samples) |
| Yield | 4–12 µg | 20–60 µg | 300–600 µg | 6 µg |
Paternity testing by RFLP analysis.
Three paternity cases tested with DNA purified from blood samples with the QIAamp DNA Blood Mini Kit. 1: mother; 2: child; 3: alleged father; 4: child + alleged father. M: markers. Each lane had 3 µg of DNA loaded. Outcome: A: alleged father excluded; B & C: alleged father confirmed.
(Data kindly provided by J. James, Gene Proof Technologies, Nashville, TN, USA.)
QIAamp Spin Column procedure.
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.
QIAamp DNA Blood Kits provide proven QIAamp technology for the purification of DNA from a variety of materials. Sample sources include:
Fresh and frozen whole blood or buffy coat
Plasma or serum
Bone marrow
Lymphocytes
Platelets
Body fluids
Cultured cells
Swabs and buccal cells
