规格
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Cell Permeability:
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Cell-Impermeant |
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Detection Method:
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Fluorescent |
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Excitation/Emission:
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multiple |
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Flow Cytometer Laser Lines:
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488, 633/635, 561, 532 |
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For Use With (Equipment):
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Flow Cytometer |
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Form:
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Solution |
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Label Type:
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Other Label(s) or Dye(s) |
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Label or Dye:
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SYTOX™ Green, SYTOX® Blue, SYTOX® Orange, SYTOX® AADvanced™, SYTOX® Red |
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Product Line:
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SYTOX® |
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Shipping Condition:
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Room Temperature |
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Format:
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Tube(s) |
储存
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Contains 5 vials total - 1 vial each of SYTOX® Blue, SYTOX® Green, SYTOX® Orange, SYTOX® Red, SYTOX® AADvanced™ dead cell stains in DMSO.
Store at ≤-20°C, desiccated and protected from light. |
SYTOX™ Dead Cell Stain Sampler Kit contains five nucleic acid stains—SYTOX™ Blue, SYTOX™ Green, SYTOX™ Orange, SYTOX™ AADvanced™, SYTOX™ Red—that can be used to determine the viability of cells in flow cytometry assays.
View a selection guide for all nonfixable viability dyes for flow cytometry.
You get these advantages with SYTOX™ Dead Cell Stains:
• Bright Signal—Easily distinguishes dead cells from live cells.
• Rapid Staining—Single step, No Wash Assay to assess viability of unfixed cells.
• Validation—in Jurkat, ReH, CHO, Kg1a, 3T3, HeLa, HEK (primary cells), and lysed whole blood.
• Optimization—Dye concentration has been optimized for use in flow cytometry.
• Versatility—Tested in a variety of experimental applications.
Try SYTOX™ Dead Cell Stains in a flexible trial size
The SYTOX™ Dead Cell Stain Sampler Kit contains a small trial size of 5 different stains (50 tests per stain) tests to help identify the best stain to work in multi-color experiments. The SYTOX™ Dead Cell Stain Sampler Kit includes the following stains:
• SYTOX™ Blue Dead Cell Stain (S34857): For the 405 nm violet laser.
• SYTOX™ Green Dead Cell Stain (S34860): For the 488 nm blue laser with green emission.
• SYTOX™ AADvanced™ Dead Cell Stain (S10274, S10309): For the 488 nm blue laser with red emission.
• SYTOX™ Red Dead Cell Stain (S34854): For the 633/5 nm red laser.
• SYTOX™ Orange Dead Cell Stain (S34861): For the 532 nm green laser.
Bright stains in an easy to use, no-wash assay
SYTOX™ Dead Cell Stains are cell impermeant nucleic acid stains. By flow cytometry, dead cells can be easily distinguished from live cells by the increased fluorescent signal due to dsDNA binding of SYTOX™ dyes (Fig 1). Because they are non-fluorescent in aqueous media, SYTOX™ Dead Cell Stains can be applied to cells and visualized with minimal background staining and without an additional wash step.
Validated in flow cytometry
The SYTOX™ Dead Cell Stains in the Sampler Kit have been optimized for use in cell concentrations ranging from 1x104 to 1x106 cells per ml.
SYTOX™ Cell Stains have been validated in several cell types including:
• Jurkat
• ReH
• CHO
• Kg1a
• 3T3
• HeLa
• HEK (primary cells)
• Lysed whole blood
SYTOX™ Dead Cell Stains have been tested in several applications including:
• Apoptosis (Fig 2)
• Cell cycle
• Cell proliferation
• Immunophenotyping assays
The SYTOX™ Family of Cell Viability Stains
SYTOX™ Dead Cell Stains Come in an assortment of excitation/emission characteristics and have been incorporated into a number of assays for apoptosis, cell viability, and metabolism.
Related Link
• Learn about all SYTOX™ Products.
