规格
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For Use With (Application):
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Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR |
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No. of Reactions:
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25 Preps |
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Sample Type:
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Bacteria |
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High-throughput Compatibility:
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Not High-throughput Compatible (Manual) |
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Prep Scale:
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100-200 µg (Medium-Scale) Plasmid DNA |
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Purification Target:
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Plasmid DNA |
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Product Line:
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GeneJET |
Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 100 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.
Highlights
• Efficient—high yields up to 200 µg of high quality plasmid DNA from 50 mL of bacterial culture
• High concentration of DNA purified—> 400 ng/ µL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns
Applications
• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription
• Transfection of robust cell lines
