QIAquick Gel Extraction Kit包含离心柱、缓冲液和收集管，用于从凝胶（多至400 mg凝胶条）或酶促反应物中纯化DNA片段。通过简单快速的结合、洗涤、洗脱步骤，使用30–50 μl洗脱体积即可纯化得到70 bp–10 kb的DNA片段。通过内置的pH指示剂可容易确定DNA结合到离心柱的最佳pH值，整个过程可在QIAcube全自动核酸纯化仪上自动化运行。
即用型DNA回收率高达95%
流程快速、方便
3个简单步骤即可回收多达10 kb DNA片段
包含凝胶上样染料，方便样本分析
| Features | Specifications |
|---|---|
| Binding capacity | 10 µg |
| Format | Tube |
| Fragment size | 70 bp – 10 kb |
| Recovery: oligonucleotides dsDNA | Recovery: dsDNA fragments |
| Processing | Manual |
| Removal <10mers 17–40mers dye terminator proteins | Removal <10mers |
| Elution volume | 30–50 µl |
| Technology | Silica technology |
| Sample type: applications | DNA: PCR reactions |
QIAquick and MinElute procedure.
The QIAquick and MinElute systems use a simple bind-wash-elute procedure with spin columns or a vacuum manifold.
High recoveries from gels.
DNA fragments (sizes indicated) before extraction with the QIAquick Gel Extraction Kit and pooled after extraction are shown. Recoveries of approximately 80% are obtained for all fragment sizes [A] 1–5: before extraction; P: pooled after extraction. Samples were analyzed on a 1.5% agarose gel in TAE buffer. [B] 1–3: before extraction; P: pooled after extraction. Samples were analyzed on a 3.5% high-resolution agarose gel in TAE buffer. M: pTZ-HinfI markers.
QIAquick系统纯化的DNA片段可直接用于各种应用，包括测序、连接和转化、限制性酶切、标记、显微注射、PCR和体外转录。
