General description
Intracellular signals mediated by receptor tyrosine kinases play a pivotal role in morphogenesis, cell fate determination and pathogenesis. c-Cbl is a ubiquitin ligase (E3) that facilitates the regulation of these pathways, attenuating signalling through ubiquitination and subsequent protein degradation. c-Cbl contains several functional domains, including a tyrosine kinase-binding domain that associates with a number of protein tyrosine kinases in a phosphorylation-dependent manner, a catalytic RING-finger domain that interacts with E2s and is essential for E3 activity and a C-terminal proline rich domain which mediates interactions with SH3 containing proteins. Mutations in the gene for c-Cbl have been implicated in a number of human cancers, particularly acute myeloid leukaemia.
N-terminal cMyc and GST-tagged, recombinant human IKKγ full length, expressed by baculovirus in Sf21 insect cells.
Application
Ubiquitination Cascade Comoponent: E3
Physical form
Purified using glutathione sepharose.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Level | 100,300 |
| recombinant | expressed in baculovirus infected Sf21 cells |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable |
| NCBI accession no. | NM_005188 |
