General description
Also known as tetratricopeptide repeat protein 8, TTC8 is a BBSome complex that is required for ciliogenesis. It is known to associate with the Rab8 GDP/GTP exchange factor, which is localized in the basal body. Rab8(GTP) is produced and enters the primary cilium, where it promotes ciliary membrane biogenesis. Mutations in TTC8 have been identified in Bardet-Biedl syndrome (BBS), a genetic disorder which is characterized with obesity, retinal degeneration, and nephropathy.
~61 kDa observed. Uncharacterized bands at ~270 kDa, ~160 kDa, and ~38 kDa may be observed in some cell lysates.
Immunogen
Epitope: TPR1 domain
KLH-conjugated linear peptide corresponding to the TPR1 domain of human TTC8.
Application
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected TTC8 in human prostate cancer and mouse eye tissues.
Research Category
Neuroscience
Research Sub Category
Cytoskeleton
Anti-TTC8 detects levels of TTC8 proteins & has been published & validated for use in WB & IHC.
Biochem/physiol Actions
Other homologies: Rat (53% sequence homology).
This antibody recognizes the TPR1 domain of TTC8.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
ARPE19 tissue lysate
Evaluated by Western Blot in ARPE19 tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected TTC8 in 10 µg of ARPE19 tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 300 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | horse, mouse, human |
| species reactivity (predicted by homology) | primate (based on 100% sequence homology), porcine (based on 100% sequence homology), equine (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_653197 |
| UniProt accession no. | Q8TAM2 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TTC8(123016) |
