Immunogen
Synthetic peptide corresponding to amino acids 439-458 of the cytoplasmic tail of human CD4.
Application
Detect CD4 using this Anti-CD4 Antibody, intracellular, clone 024-10D6.B3 validated for use in ELISA, EMSA, FUNC & IC.
Immunocytochemistry
ELISA
Stimulation/Inhibition of biological activity
Electrophoretic mobility shift analysis
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes the cytoplasmic tail of CD4. Many functions have been assigned to this tail, including anti-viral regulation (down/up regulation of CD4), T-cell maturation, activation and differentiation, and Lck signal transduction, among others. This antibody is useful as a control for studies involving targeting of intracellular protein domains of T cells.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.02 M phosphate buffer, 0.25M NaCl, with 0.1% sodium azide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| clone | 024-10D6.B3, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,electrophoretic mobility shift assay: suitable,immunocytochemistry: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_000616.3 |
| UniProt accession no. | P01730 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
