General description
The protein named ORM1-like protein 3 (ORMDL3), and encoded by the human gene ORMDL3 belongs to a novel gene family of endoplasmic reticulum membrane proteins that are highly conserved and ubiquitously expressed in adult and fetal tissues. ORMDL3 is a negative regulator of sphingolipid biosynthesis and may indirectly regulate endoplasmic reticulum-mediated calcium signaling. Also in lung tissue, ORMDL3 is an inducible epithelial protein that regulates metalloproteases, chemokines, oligoadenylate synthetases and activates activating transcription factor 6 (ATF6), a critical protein in the unfolded protein response immune response pathway which plays an important role in our response to infections and pathology. The discovery of the link between ORMDL3 and the unfolded protein response pathway may be part of the mechanism of how ORMDL3 is involved in the asthmatic response and regulation. Defects and polymorphims in ORMDL3 gene are linked with Asthma and with asthmatic response profiles.
~19 kDa observed
Immunogen
Blue carrier protein-conjugated linear peptide corresponding to human near C-Terminal of ORMDL3.
Epitope: Near C-Terminal
Application
Anti-ORMDL3 Antibody detects level of ORMDL3 & has been published & validated for use in ORMDL3.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected ORMDL3 in human thalamus and human lung tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected ORMDL3 in 10 µg of human brain tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | horse (based on 100% sequence homology), monkey (based on 100% sequence homology), canine (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_644809 |
| UniProt accession no. | Q8N138 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ORMDL3(94103) |
