General description
Histone H2A deubiquitinase MYSM, often referred to as 2A-DUB is metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A. 2A-DUB regulates transcription by coordinating histone acetylation and deubiquitination, and destabilizing the association of linker histone H1 with nucleosomes. The 2A-DUB mechanism may just be one of many H2A ubiquitination proteins that have the ability to regulate transcription. 2A-DUB is also essential for cell cycle progression and gene expression.
~100 kDa observed. Uncharacterized bands at ~26, ~35, ~49 and ~66 kDa may be observed in in some lysates. Bands at ~66 and ~26 kDa may represent isoforms resulting from alternative splicing.
Immunogen
Recombinant protein corresponding to human of MYSM1.
Application
Anti-MYSM1/2A-DUB Antibody is a Rabbit Polyclonal Antibody for detection of MYSM1/2A-DUB also known as Histone H2A deubiquitinase MYSM1 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
NIH/3T3 cell lysate
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected MYSM1 on 10 µg of NIH/3T3 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human, mouse |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_001078956 |
| UniProt accession no. | Q5VVJ2 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MYSM1(114803) |
